Hotspot

rpl20-rps12

LSC · 71711-72477 · 767 bp

Back to Chloranthus

Pi 0.0162

MarkerSeek score 61.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 27
pi: 0.0162
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AGGAACGTGTACTAGGGTGT	50.0	50.0	57.9	57.7	868-909 bp	1.0	80.6
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AGGAACGTGTACTAGGGTGT	50.0	50.0	57.9	57.7	867-908 bp	1.0	80.6
rpl20-rps12_p3	TTTTTGTTCGACGTCTCCGA	AGGAACGTGTACTAGGGTGT	45.0	50.0	57.8	57.7	828-869 bp	1.0	80.0
rpl20-rps12_p4	ATCCCCGTCTAACTCTGGTC	AGGAACGTGTACTAGGGTGT	55.0	50.0	58.2	57.7	801-842 bp	1.0	80.0
rpl20-rps12_p5	TTCGACGTCTCCGAGCTATA	AGGAACGTGTACTAGGGTGT	50.0	50.0	57.8	57.7	822-863 bp	1.0	79.9