Hotspot

rpl33-rps18

LSC · 70626-70780 · 155 bp

Back to Chloranthus

Pi 0.0337

MarkerSeek score 62.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 12
pi: 0.0337
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGAATCGGCACAATACACCT	CGAAAAGGTCGCTTAGATTT	50.0	40.0	58.0	54.0	271-305 bp	1.0	52.7
rpl33-rps18_p2	AGGAATCGGCACAATACACC	CGAAAAGGTCGCTTAGATTT	50.0	40.0	58.0	54.0	272-306 bp	1.0	52.6
rpl33-rps18_p3	AGGAATCGGCACAATACACC	CGAAAAGGTCGCTTAGATTTATCC	50.0	41.7	58.0	58.0	272-306 bp	1.0	52.1
rpl33-rps18_p4	GGAATCGGCACAATACACCT	CGAAAAGGTCGCTTAGATTTATCC	50.0	41.7	58.0	58.0	271-305 bp	1.0	52.1
rpl33-rps18_p5	ACTCAAAGGAATCGGCACAA	CGAAAAGGTCGCTTAGATTT	45.0	40.0	57.7	54.0	278-312 bp	1.0	50.7