Hotspot

ycf4-cemA

LSC · 63164-64101 · 938 bp

Back to Chloranthus

Pi 0.0213

MarkerSeek score 63.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 43
pi: 0.0213
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AAATCCACCAGGGCAACAAT	45.0	45.0	58.1	58.0	1063-1095 bp	1.0	85.5
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AGATATGGGAATGGGAGGGG	45.0	55.0	58.1	57.9	1031-1063 bp	1.0	85.4
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	AAATCCACCAGGGCAACAAT	45.0	45.0	57.8	58.0	1077-1109 bp	1.0	84.8
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	ATCCACCAGGGCAACAATAC	45.0	50.0	58.1	57.9	1061-1093 bp	1.0	84.6
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	AGATATGGGAATGGGAGGGG	45.0	55.0	57.8	57.9	1045-1077 bp	1.0	84.6