Hotspot

psbZ-trnG-GCC

LSC · 37498-37851 · 354 bp

Back to Chloranthus

Pi 0.0344

MarkerSeek score 71.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 27
pi: 0.0344
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GGTACGTCGTTATGGATTGGA	TACATCACCATGGGCGGATA	47.6	50.0	57.6	58.3	458-514 bp	1.0	74.6
psbZ-trnG-GCC_p2	GGTACGTCGTTATGGATTGGA	GATCGAACCCGCATCTTCTC	47.6	55.0	57.6	58.5	433-489 bp	1.0	73.0
psbZ-trnG-GCC_p3	GGTACGTCGTTATGGATTGGA	GAACCCGCATCTTCTCCTTG	47.6	55.0	57.6	58.6	429-485 bp	1.0	72.1
psbZ-trnG-GCC_p4	GGTACGTCGTTATGGATTGGA	TACCATTCGACCATATCCGC	47.6	50.0	57.6	57.3	395-451 bp	1.0	71.6
psbZ-trnG-GCC_p5	GGTACGTCGTTATGGATTGGA	TATACATCACCATGGGCGGA	47.6	50.0	57.6	58.3	502 bp	0.285714	37.7