Hotspot

trnD-GUC-trnY-GUA

LSC · 31512-31857 · 346 bp

Back to Chloranthus

Pi 0.0447

MarkerSeek score 69.40

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 29
pi: 0.0447
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TTCATCGGATCCTAGGTCGG	GGGGACGGACTGTAAATTCG	55.0	55.0	58.4	58.4	449-498 bp	1.0	83.0
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	388-437 bp	1.0	83.0
trnD-GUC-trnY-GUA_p3	TTCATCGGATCCTAGGTCGG	GGGACGGACTGTAAATTCGT	55.0	50.0	58.4	57.6	448-497 bp	1.0	82.8
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	387-436 bp	1.0	82.8
trnD-GUC-trnY-GUA_p5	TTCATCGGATCCTAGGTCGG	GATTCTTCCTGGGTCGATGC	55.0	55.0	58.4	58.4	482-531 bp	1.0	82.2