Hotspot

rpoB-trnC-GCA

LSC · 27519-28691 · 1173 bp

Back to Chloranthus

Pi 0.0188

MarkerSeek score 68.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 51
pi: 0.0188
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CTGGGGAGAAAGGATTTGCA	50.0	50.0	57.3	57.8	1208-1262 bp	1.0	79.6
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	TGGGGAGAAAGGATTTGCAG	50.0	50.0	57.3	57.8	1207-1261 bp	1.0	79.6
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	ACTGGGGAGAAAGGATTTGC	50.0	50.0	57.3	57.8	1209-1263 bp	1.0	79.6
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	AGAAAGGATTTGCAGTCCCC	50.0	50.0	57.3	57.8	1202-1256 bp	1.0	79.6
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCG	GGGGAGAAAGGATTTGCAGT	50.0	50.0	57.3	57.8	1206-1260 bp	1.0	79.6