Hotspot

atpI-rps2

LSC · 15880-16237 · 358 bp

Back to Chloranthus

Pi 0.0537

MarkerSeek score 67.60

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 358
variable_sites: 27
pi: 0.0537
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	GCGATTACGACCCAAGAAGT	CTTTGGGGATTCCGACGATT	50.0	50.0	58.0	58.0	530-668 bp	1.0	88.2
atpI-rps2_p2	GCGATTACGACCCAAGAAGT	ATCAGCTGCAATAAGGGCTC	50.0	50.0	58.0	58.0	820-958 bp	1.0	87.0
atpI-rps2_p3	GCGATTACGACCCAAGAAGT	GGGCTCGGTGTCATTATGTT	50.0	50.0	58.0	58.0	806-944 bp	1.0	86.9
atpI-rps2_p4	GGAAACCCCCTATTTGCCAA	ATCAGCTGCAATAAGGGCTC	50.0	50.0	58.1	58.0	777-915 bp	1.0	86.7
atpI-rps2_p5	TTGGAAACCCCCTATTTGCC	ATCAGCTGCAATAAGGGCTC	50.0	50.0	58.1	58.0	779-917 bp	1.0	86.7