Hotspot

atpF-atpH

LSC · 13660-14130 · 471 bp

Back to Chloranthus

Pi 0.0575

MarkerSeek score 82.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 65
pi: 0.0575
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTAACCCACGGAAACAA	AGCAGAGGGTAAAATACGCG	50.0	50.0	58.0	58.1	573-650 bp	1.0	91.2
atpF-atpH_p2	CCAGTAACCCACGGAAACAA	AATTTACGGACTGGTCGTGG	50.0	50.0	58.0	57.9	513-590 bp	1.0	91.0
atpF-atpH_p3	CCAGTAACCCACGGAAACAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.0	58.3	499-576 bp	1.0	89.6
atpF-atpH_p4	CCAGTAACCCACGGAAACAA	CAGAGGGTAAAATACGCGGT	50.0	50.0	58.0	57.7	571-648 bp	1.0	89.3
atpF-atpH_p5	GACCAGTAACCCACGGAAAC	AGCAGAGGGTAAAATACGCG	55.0	50.0	58.5	58.1	575-652 bp	1.0	87.6