Hotspot

ndhF

SSC · 111236-113458 · 2223 bp

Back to Chionographis

Pi 0.0093

MarkerSeek score 48.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 31
pi: 0.0093
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGGGTGAATGGAAAGGAAA	GCCGTGCCATCCCTATAATT	50.0	50.0	58.1	57.8	2933-2949 bp	1.0	79.4
ndhF_p2	AAGAGATCCGGGTGAATGGA	GCCGTGCCATCCCTATAATT	50.0	50.0	58.1	57.8	2940-2956 bp	1.0	79.1
ndhF_p3	AGAGATCCGGGTGAATGGAA	GCCGTGCCATCCCTATAATT	50.0	50.0	58.1	57.8	2939-2955 bp	1.0	79.1
ndhF_p4	GAAGAGATCCGGGTGAATGG	GCCGTGCCATCCCTATAATT	55.0	50.0	57.8	57.8	2941-2957 bp	1.0	78.1
ndhF_p5	GGAAGAGATCCGGGTGAATG	GCCGTGCCATCCCTATAATT	55.0	50.0	57.8	57.8	2942-2958 bp	1.0	78.1