Hotspot

rpl32-trnL-UAG

SSC · 114375-115237 · 863 bp

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Pi 0.0145

MarkerSeek score 61.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 863
variable_sites: 15
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTGTGCGACAAACAAGT	AGCGTGTCTACCGATTTCAC	35.0	50.0	55.2	58.0	882-930 bp	1.0	62.5
rpl32-trnL-UAG_p2	TTTTTGTGCGACAAACAAGT	CAGCGTGTCTACCGATTTCA	35.0	50.0	55.2	58.0	883-931 bp	1.0	62.5
rpl32-trnL-UAG_p3	TTTTTGTGCGACAAACAAGT	GTCCCTTTTAGGAGATGCCG	35.0	55.0	55.2	58.0	948-996 bp	1.0	62.1
rpl32-trnL-UAG_p4	TTTTTGTGCGACAAACAAGT	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	55.2	57.9	892-940 bp	1.0	61.6
rpl32-trnL-UAG_p5	TTTTTGTGCGACAAACAAGT	CTAAGAGCAGCGTGTCTACC	35.0	55.0	55.2	57.8	890-938 bp	1.0	61.0