Hotspot

rps12-trnV-GAC

IRb · 99487-101296 · 1810 bp

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Pi 0.00371

MarkerSeek score 61.50

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1810
variable_sites: 10
pi: 0.00371
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	45.0	50.0	57.7	57.9	1967-2008 bp	1.0	78.2
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	45.0	50.0	57.7	57.8	1906-1947 bp	1.0	77.9
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	45.0	50.0	57.7	57.8	1910-1951 bp	1.0	77.8
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	45.0	50.0	57.7	57.8	1905-1946 bp	1.0	77.8
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	45.0	55.0	57.7	58.3	1908-1949 bp	1.0	76.9