Hotspot

psbT-psbN

LSC · 74644-74703 · 60 bp

Back to Chionographis

Pi 0.0778

MarkerSeek score 68.60

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 7
pi: 0.0778
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	CCATTTGAAGAACACGGGGA	50.0	50.0	57.9	58.1	127 bp	1.0	82.9
psbT-psbN_p2	TTTTCGAGAACCGCCTAAGG	TCCATTTGAAGAACACGGGG	50.0	50.0	57.9	58.1	128 bp	1.0	82.9
psbT-psbN_p3	CGCCTAAGGTTCCGACTAAA	CCATTTGAAGAACACGGGGA	50.0	50.0	57.3	58.1	116 bp	1.0	78.8
psbT-psbN_p4	CGCCTAAGGTTCCGACTAAA	TCCATTTGAAGAACACGGGG	50.0	50.0	57.3	58.1	117 bp	1.0	78.7
psbT-psbN_p5	TTTTCGAGAACCGCCTAAGG	ATTTGAAGAACACGGGGACT	50.0	45.0	57.9	57.0	125 bp	1.0	76.2