Hotspot

rpl20-rps12

LSC · 69285-70061 · 777 bp

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Pi 0.00603

MarkerSeek score 50.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 7
pi: 0.00603
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCTCGTCTAACTCTGGTCA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.1	58.7	826-828 bp	1.0	68.1
rpl20-rps12_p2	TCCTCGTCTAACTCTGGTCA	AGCGTCGAGGAACATGTACT	50.0	50.0	57.1	58.8	825-827 bp	1.0	67.4
rpl20-rps12_p3	TCCTCGTCTAACTCTGGTCA	TCAGCGTCGAGGAACATGTA	50.0	50.0	57.1	58.8	827-829 bp	1.0	67.3
rpl20-rps12_p4	TCCTCGTCTAACTCTGGTCA	GCGTCGAGGAACATGTACTA	50.0	50.0	57.1	57.2	824-826 bp	1.0	67.3
rpl20-rps12_p5	TCCTCGTCTAACTCTGGTCAT	CAGCGTCGAGGAACATGTAC	47.6	55.0	57.6	58.7	826-828 bp	1.0	64.2