Hotspot

petA-psbJ

LSC · 63205-64305 · 1101 bp

Back to Chionographis

Pi 0.0143

MarkerSeek score 59.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 23
pi: 0.0143
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAATTGTACGA	GGTTCATATTCCGGGTTGGG	33.3	55.0	53.7	58.3	1168-1178 bp	1.0	42.2
petA-psbJ_p2	AGAAACAGTTTGAAAAGGTTCA	GGTTCATATTCCGGGTTGGG	31.8	55.0	54.6	58.3	1178-1188 bp	1.0	42.2
petA-psbJ_p3	TGAAAAGGTTCAATTGTACGAA	GGTTCATATTCCGGGTTGGG	31.8	55.0	54.5	58.3	1168-1178 bp	1.0	42.2
petA-psbJ_p4	TTGAAAAGGTTCAATTGTACGA	GGTTCATATTCCGGGTTGGG	31.8	55.0	54.5	58.3	1169-1179 bp	1.0	42.2
petA-psbJ_p5	AGGTTCAATTGTACGAAATGA	GGTTCATATTCCGGGTTGGG	33.3	55.0	53.5	58.3	1163-1173 bp	1.0	42.2