Hotspot

trnF-GAA-ndhJ

LSC · 48721-49468 · 748 bp

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Pi 0.00536

MarkerSeek score 63.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 6
pi: 0.00536
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGTTGGGATAGCTCAGTTGG	TCTTGATGCCCGAAAGTTGG	55.0	50.0	58.0	58.5	900-908 bp	1.0	77.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TCTTGATGCCCGAAAGTTGG	50.0	50.0	58.1	58.5	860-868 bp	1.0	77.1
trnF-GAA-ndhJ_p3	GGTTGGGATAGCTCAGTTGG	AAACGTATCTTGATGCCCGA	55.0	45.0	58.0	57.3	907-915 bp	1.0	75.4
trnF-GAA-ndhJ_p4	GGTTGGGATAGCTCAGTTGG	AACGTATCTTGATGCCCGAA	55.0	45.0	58.0	57.3	906-914 bp	1.0	75.4
trnF-GAA-ndhJ_p5	GGTTGGGATAGCTCAGTTGG	ACGTATCTTGATGCCCGAAA	55.0	45.0	58.0	57.3	905-913 bp	1.0	75.4