Hotspot

ycf1

SSC · 125386-131004 · 5619 bp

Back to Chionanthus

Pi 0.0138

MarkerSeek score 56.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5619
variable_sites: 430
pi: 0.0138
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TATCCCAGGCCTCTGCTATC	CATGCCAAAGTGCTGGAAAG	55.0	50.0	58.1	57.9	1224-1236 bp	1.0	87.5
ycf1_p2	ACGCCTTTGAGCACGTATAG	CATGCCAAAGTGCTGGAAAG	50.0	50.0	58.1	57.9	959-971 bp	1.0	87.5
ycf1_p3	TTTTGAATGCTGACCCCCTC	CATGCCAAAGTGCTGGAAAG	50.0	50.0	58.1	57.9	1110-1122 bp	1.0	87.4
ycf1_p4	TCTCTATTTGCGCGTTCTCC	CATGCCAAAGTGCTGGAAAG	50.0	50.0	58.1	57.9	1206-1218 bp	1.0	87.4
ycf1_p5	CTCTATTTGCGCGTTCTCCT	CATGCCAAAGTGCTGGAAAG	50.0	50.0	58.1	57.9	1205-1217 bp	0.947368	85.3