Hotspot

ndhF

SSC · 112128-114344 · 2217 bp

Back to Chionanthus

Pi 0.0119

MarkerSeek score 55.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 149
pi: 0.0119
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGATCCGCCGAAGAAGATC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2485-2501 bp	1.0	77.3
ndhF_p2	GCAGAATACCGTCACCCATT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.0	58.0	2621-2637 bp	1.0	77.2
ndhF_p3	GCCGAAGAAGATCCTTCTCC	CGCTGTCCCATATCCTTTCC	55.0	55.0	57.8	58.1	2991-3000 bp	0.526316	63.0
ndhF_p4	CGCCGAAGAAGATCCTTCTC	CGCTGTCCCATATCCTTTCC	55.0	55.0	58.2	58.1	2992-3000 bp	0.421053	58.8
ndhF_p5	GCCGAAGAAGATCCTTCTCC	ACGCTGTCCCATATCCTTTC	55.0	50.0	57.8	57.4	2953-3000 bp	0.473684	57.9