Hotspot

ndhF-rpl32

SSC · 114345-114822 · 478 bp

Back to Chionanthus

Pi 0.0186

MarkerSeek score 70.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 478
variable_sites: 49
pi: 0.0186
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCCACGCATATTGATATGTCTGT	TGCTTTTAACGCTGTCCCAT	39.1	45.0	57.8	58.1	552-605 bp	1.0	64.8
ndhF-rpl32_p2	TCCACGCATATTGATATGTCTGT	ATGCTTTTAACGCTGTCCCA	39.1	45.0	57.8	58.1	553-606 bp	1.0	64.7
ndhF-rpl32_p3	GGTATGATCCACGCATATTGA	ATGCTTTTAACGCTGTCCCA	42.9	45.0	55.6	58.1	560-613 bp	1.0	63.4
ndhF-rpl32_p4	GGTATGATCCACGCATATTGA	TGCTTTTAACGCTGTCCCAT	42.9	45.0	55.6	58.1	559-612 bp	1.0	63.4
ndhF-rpl32_p5	TCCACGCATATTGATATGTCTGT	CGCTGTCCCATATCCTTTCC	39.1	55.0	57.8	58.1	583-596 bp	0.947368	59.8