Hotspot

psbE-petL

LSC · 67142-68324 · 1183 bp

Back to Chionanthus

Pi 0.0154

MarkerSeek score 70.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 96
pi: 0.0154
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGAAGTTATAGTTAAAGCCGC	50.0	43.5	57.9	58.2	1309-1349 bp	0.947368	61.8
psbE-petL_p2	ACTGACGAATAACCAACCCG	GCTGAAGTTATAGTTAAAGCCGC	50.0	43.5	57.9	58.2	1322-1362 bp	0.947368	61.4
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCTGAAGTTATAGTTAAAGCCGC	50.0	43.5	57.8	58.2	1232-1272 bp	0.947368	61.3
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GCTGAAGTTATAGTTAAAGCCGC	45.0	43.5	58.2	58.2	1318-1358 bp	0.947368	60.8
psbE-petL_p5	CGAATAACCAACCCGCAATG	GCTGAAGTTATAGTTAAAGCCGC	50.0	43.5	57.8	58.2	1317-1357 bp	0.947368	60.7