Hotspot

petA-psbJ

LSC · 65413-66360 · 948 bp

Back to Chionanthus

Pi 0.0116

MarkerSeek score 75.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 67
pi: 0.0116
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1072-1106 bp	1.0	85.8
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	986-1020 bp	1.0	85.0
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1055-1089 bp	1.0	84.9
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1069-1103 bp	1.0	84.6
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	985-1019 bp	1.0	84.1