Hotspot

ndhC-trnM(CAU)

LSC · 52387-54326 · 1940 bp

Back to Chionanthus

Pi 0.0118

MarkerSeek score 66.50

Primer available yes

Label ndhC-trnM(CAU)

Feature Metadata

label_name: ndhC-trnM(CAU)
feature_type: igs
region: LSC
length_bp: 1940
variable_sites: 139
pi: 0.0118
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM(CAU)_p1	CGGAAATGAAAAATGCCAAA	CCCGCCGTATGAAAGCAATA	35.0	50.0	53.6	58.4	2052-2081 bp	1.0	48.7
ndhC-trnM(CAU)_p2	CGGAAATGAAAAATGCCAAAA	CCCGCCGTATGAAAGCAATA	33.3	50.0	54.5	58.4	2052-2081 bp	1.0	47.7
ndhC-trnM(CAU)_p3	CGGAAATGAAAAATGCCAAA	AGGTACCATTGACTCCGGTA	35.0	50.0	53.6	57.5	2113-2142 bp	1.0	47.5
ndhC-trnM(CAU)_p4	CGGAAATGAAAAATGCCAAA	TAGGTACCATTGACTCCGGT	35.0	50.0	53.6	57.5	2114-2143 bp	1.0	47.5
ndhC-trnM(CAU)_p5	CGGAAATGAAAAATGCCAAA	CCGCCGTATGAAAGCAATAC	35.0	50.0	53.6	57.3	2051-2080 bp	1.0	47.4