Hotspot

trnT(UGU)-trnL(UAA)

LSC · 48421-49073 · 653 bp

Back to Chionanthus

Pi 0.0123

MarkerSeek score 70.50

Primer available yes

Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name: trnT(UGU)-trnL(UAA)
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 48
pi: 0.0123
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(UGU)-trnL(UAA)_p1	TGAAGGAAAAGCCGGCTATC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	754-791 bp	1.0	85.4
trnT(UGU)-trnL(UAA)_p2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	702-739 bp	1.0	85.0
trnT(UGU)-trnL(UAA)_p3	TGAAGGAAAAGCCGGCTATC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	762-799 bp	1.0	84.8
trnT(UGU)-trnL(UAA)_p4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	710-747 bp	1.0	84.4
trnT(UGU)-trnL(UAA)_p5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	734-771 bp	1.0	83.9