Hotspot

ycf3-trnS(GGA)

LSC · 46226-46999 · 774 bp

Back to Chionanthus

Pi 0.00997

MarkerSeek score 70.70

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 57
pi: 0.00997
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	877-898 bp	1.0	76.9
ycf3-trnS(GGA)_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	848-869 bp	1.0	76.4
ycf3-trnS(GGA)_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	898-919 bp	1.0	76.3
ycf3-trnS(GGA)_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	878-899 bp	1.0	74.6
ycf3-trnS(GGA)_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	875-896 bp	1.0	73.9