Hotspot

trnE(UUC)-psbD

LSC · 32386-34555 · 2170 bp

Back to Chionanthus

Pi 0.00925

MarkerSeek score 72.90

Primer available yes

Label trnE(UUC)-psbD

Feature Metadata

label_name: trnE(UUC)-psbD
feature_type: igs
region: LSC
length_bp: 2170
variable_sites: 129
pi: 0.00925
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(UUC)-psbD_p1	TTTCGTAGTACCCTACCCCC	GGACCAGCCTACAAAAACGA	55.0	50.0	57.8	58.1	2340-2380 bp	1.0	86.1
trnE(UUC)-psbD_p2	GCTGCCTCCTTGAAAGAGAG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.3	58.1	2302-2342 bp	1.0	85.3
trnE(UUC)-psbD_p3	TTTCGTAGTACCCTACCCCC	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.8	58.2	2330-2370 bp	1.0	85.1
trnE(UUC)-psbD_p4	GCTGCCTCCTTGAAAGAGAG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.3	58.2	2292-2332 bp	1.0	84.3
trnE(UUC)-psbD_p5	TTTCGTAGTACCCTACCCCC	CAAAAACGAAACGGTCCCTC	55.0	50.0	57.8	57.6	2329-2369 bp	1.0	83.9