Hotspot

rps2-rpoC2

LSC · 17452-17658 · 207 bp

Back to Chionanthus

Pi 0.0249

MarkerSeek score 79.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 21
pi: 0.0249
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	365-383 bp	1.0	86.3
rps2-rpoC2_p2	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.1	57.8	359-377 bp	1.0	86.3
rps2-rpoC2_p3	TCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.8	57.8	362-380 bp	1.0	85.7
rps2-rpoC2_p4	GAACTCCTGCCTCCATCATC	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.7	57.8	366-384 bp	1.0	85.1
rps2-rpoC2_p5	CTCCTGCCTCCATCATCTCT	TTCGAGGGGGAAATGAGAGA	55.0	50.0	58.3	57.8	363-381 bp	1.0	84.9