Hotspot

psbI-trnS(GCU)

LSC · 9228-9349 · 122 bp

Back to Chionanthus

Pi 0.108

MarkerSeek score 76.10

Primer available yes

Label psbI-trnS(GCU)

Feature Metadata

label_name: psbI-trnS(GCU)
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 32
pi: 0.108
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS(GCU)_p1	GTAATCCCGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.3	58.0	208-232 bp	1.0	86.7
psbI-trnS(GCU)_p2	GTAATCCCGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.3	58.0	209-233 bp	1.0	86.7
psbI-trnS(GCU)_p3	GTAATCCCGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.3	58.1	227-251 bp	1.0	85.8
psbI-trnS(GCU)_p4	GTAATCCCGGACGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.3	58.4	206-230 bp	1.0	83.9
psbI-trnS(GCU)_p5	GTAATCCCGGACGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.3	58.4	213-237 bp	1.0	83.7