Hotspot

matK-rps16

LSC · 3656-5308 · 1653 bp

Back to Chionanthus

Pi 0.0138

MarkerSeek score 62.90

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1653
variable_sites: 122
pi: 0.0138
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GGATGGGGTGGGGTATTAGT	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.2	58.0	2746-2838 bp	1.0	88.4
matK-rps16_p2	TGGATGGGGTGGGGTATTAG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.2	58.0	2747-2839 bp	1.0	88.4
matK-rps16_p3	GGATGGGGTGGGGTATTAGT	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.2	57.9	2332-2419 bp	1.0	87.5
matK-rps16_p4	TGGATGGGGTGGGGTATTAG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.2	57.9	2333-2420 bp	1.0	87.5
matK-rps16_p5	GCGCCCCCAAAATTGATTTT	AAAAGGGTATGTTGCTGCCA	45.0	45.0	58.2	58.0	2535-2624 bp	0.789474	79.0