Hotspot

trnH(GUG)-psbA

LSC · 92-537 · 446 bp

Back to Chionanthus

Pi 0.0472
MarkerSeek score 85.30
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
446
variable_sites
104
pi
0.0472
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 403-596 bp 1.0 88.2
trnH(GUG)-psbA_p2 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 404-597 bp 1.0 88.0
trnH(GUG)-psbA_p3 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 404-597 bp 1.0 87.7
trnH(GUG)-psbA_p4 ACTGCCTTAATCCACTTGGC ACCGTGCTAACCTTGGTATG 50.0 50.0 58.2 57.6 405-598 bp 1.0 87.5
trnH(GUG)-psbA_p5 AATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 50.0 55.0 57.7 58.3 396-589 bp 1.0 86.7