Hotspot

psbA

LSC · 87-1148 · 1062 bp

Back to Chimonocalamus

Pi 0.000858

MarkerSeek score 32.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.000858
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTCTGGCTTTCCTTCCTTCA	AATACGTGTGCTTGGGAGTC	45.0	50.0	56.7	57.9	1160 bp	1.0	68.7
psbA_p2	TTTCTGGCTTTCCTTCCTTCA	AATACGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.9	1161 bp	1.0	66.8
psbA_p3	TCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	45.0	50.0	56.7	57.9	1159 bp	0.9	64.7
psbA_p4	TCTGGCTTTCCTTCCTTCAAA	AATACGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.9	1159 bp	0.9	62.7
psbA_p5	TTCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.9	1160 bp	0.9	62.7