Hotspot

atpF

LSC · 35188-36609 · 1422 bp

Back to Chimonocalamus

Pi 0.00396

MarkerSeek score 41.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 16
pi: 0.00396
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGAGGATATGCTCGCCTTCT	CATCAACCCATCGCCCATTA	50.0	50.0	58.0	58.0	2000-2058 bp	1.0	82.6
atpF_p2	GTATTGCGAGACAGCCAGAA	CATCAACCCATCGCCCATTA	50.0	50.0	58.0	58.0	2318-2376 bp	1.0	82.2
atpF_p3	AGAGGATATGCTCGCCTTCT	TTTTGCCAGTCTGTCTGTCC	50.0	50.0	58.0	58.0	2283-2341 bp	1.0	82.2
atpF_p4	AGAGGATATGCTCGCCTTCT	CAATTTCGCCTCTCCCATCA	50.0	50.0	58.0	58.0	2121-2179 bp	1.0	82.2
atpF_p5	GTATTGCGAGACAGCCAGAA	TTTTGCCAGTCTGTCTGTCC	50.0	50.0	58.0	58.0	2601-2659 bp	1.0	81.9