Hotspot

psaC-ndhE

SSC · 112305-112785 · 481 bp

Back to Chimonocalamus

Pi 0.00494

MarkerSeek score 48.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 481
variable_sites: 8
pi: 0.00494
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTGCTTGGCTTTACACCCAT	TTTTGTTATAGCCCTTGCGG	45.0	45.0	58.0	56.4	702-720 bp	1.0	68.9
psaC-ndhE_p2	GTTGGACAAGCTCGTACACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	656-674 bp	1.0	68.0
psaC-ndhE_p3	TGTTGGACAAGCTCGTACAC	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	657-675 bp	1.0	68.0
psaC-ndhE_p4	CTGTTGGACAAGCTCGTACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	57.9	56.4	658-676 bp	1.0	67.9
psaC-ndhE_p5	CATCTGTTGGACAAGCTCGT	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.2	56.4	661-679 bp	1.0	67.5