Hotspot

rpl32-trnL-UAG

SSC · 108418-109119 · 702 bp

Back to Chimonocalamus

Pi 0.00627
MarkerSeek score 40.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
702
variable_sites
10
pi
0.00627
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-802 bp 0.9 74.9
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 773-801 bp 0.9 74.9
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 767-795 bp 0.9 74.4
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 766-794 bp 0.9 74.4
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 58.1 58.2 787-815 bp 0.9 74.4