Hotspot

psaA-ycf3

LSC · 43756-44406 · 651 bp

Back to Chimonocalamus

Pi 0.00256

MarkerSeek score 55.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 6
pi: 0.00256
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	732-762 bp	1.0	79.8
psaA-ycf3_p2	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	804-834 bp	1.0	79.6
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	728-758 bp	1.0	79.5
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	800-830 bp	1.0	79.3
psaA-ycf3_p5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.7	58.0	785-815 bp	1.0	78.2