Hotspot

trnD-GUC-psbM

LSC · 17590-18584 · 995 bp

Back to Chimonocalamus

Pi 0.00132
MarkerSeek score 53.90
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
995
variable_sites
4
pi
0.00132
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCA 50.0 40.0 58.5 54.5 1070-1081 bp 1.0 49.3
trnD-GUC-psbM_p2 TAGGTATGCCATACACCCCG TGCGAGAATATTGACTTCCA 55.0 40.0 58.7 54.5 1102-1113 bp 1.0 47.8
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATGCGAGAATATTGACTTCCA 50.0 38.1 58.5 55.1 1071-1082 bp 1.0 46.2
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCAT 50.0 38.1 58.5 55.1 1070-1081 bp 1.0 46.2
trnD-GUC-psbM_p5 GTTCAATTGGTCAGAGCACC TGCGAGAATATTGACTTCCA 50.0 40.0 57.0 54.5 1071-1082 bp 1.0 45.1