Hotspot

trnG-UCC-trnT-GGU

LSC · 14368-16337 · 1970 bp

Back to Chimonocalamus

Pi 0.00393

MarkerSeek score 53.70

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1970
variable_sites: 25
pi: 0.00393
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	38.1	52.4	55.5	58.1	2101-2104 bp	1.0	43.5
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	38.1	47.6	55.5	57.8	2114-2117 bp	1.0	42.8
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	38.1	47.6	55.5	57.8	2115-2118 bp	1.0	42.8
trnG-UCC-trnT-GGU_p4	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	2104-2107 bp	1.0	42.4
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	2103-2106 bp	1.0	42.4