Hotspot

rps16-trnQ-UUG

LSC · 5613-7164 · 1552 bp

Back to Chimonocalamus

Pi 0.00265
MarkerSeek score 57.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1552
variable_sites
14
pi
0.00265
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1607-1646 bp 1.0 80.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1618-1657 bp 1.0 79.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1611-1650 bp 1.0 79.2
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1622-1661 bp 1.0 78.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAAACCAGAAATGGGGCGT 45.0 45.0 58.1 57.7 1667-1706 bp 1.0 78.5