Hotspot

ycf3

LSC · 44391-46385 · 1995 bp

Back to Chimonobambusa

Pi 0.000456

MarkerSeek score 50.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1995
variable_sites: 5
pi: 0.000456
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGA	45.0	45.0	57.8	55.7	2137-2144 bp	0.909091	57.0
ycf3_p2	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGA	45.0	45.0	57.8	55.7	2136-2143 bp	0.909091	57.0
ycf3_p3	ATCAACAACCAAACCACCCA	AGAGCATATGGATCGATCGAT	45.0	42.9	57.8	56.3	2137-2144 bp	0.909091	53.5
ycf3_p4	TCAACAACCAAACCACCCAT	AGAGCATATGGATCGATCGAT	45.0	42.9	57.8	56.3	2136-2143 bp	0.909091	53.5
ycf3_p5	GATCAACAACCAAACCACCC	AGAGCATATGGATCGATCGA	50.0	45.0	57.2	55.7	2138-2145 bp	0.909091	51.8