Hotspot

rps19

IRa · 139286-139567 · 282 bp

Back to Chimonobambusa

Pi 0

MarkerSeek score 21.50

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRa
length_bp: 282
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGACCTCCATACTTCGATCGA	TGAATCTTTCTATTCTGAATTCAG	47.6	29.2	58.1	52.9	380 bp	1.0	40.0
rps19_p2	ACCTCCATACTTCGATCGAGA	TGAATCTTTCTATTCTGAATTCAG	47.6	29.2	57.8	52.9	378 bp	1.0	40.0
rps19_p3	CTGACCTCCATACTTCGATCG	TGAATCTTTCTATTCTGAATTCAG	52.4	29.2	57.7	52.9	381 bp	1.0	40.0
rps19_p4	TGACCTCCATACTTCGATCGA	TTGAATCTTTCTATTCTGAATTCAG	47.6	28.0	58.1	53.7	381 bp	1.0	40.0
rps19_p5	ACCTCCATACTTCGATCGAGA	TTGAATCTTTCTATTCTGAATTCAG	47.6	28.0	57.8	53.7	379 bp	1.0	40.0