Hotspot

rpl32-trnL-UAG

SSC · 108401-109102 · 702 bp

Back to Chimonobambusa

Pi 0.00378
MarkerSeek score 50.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
702
variable_sites
9
pi
0.00378
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-775 bp 1.0 78.9
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 773-774 bp 1.0 78.9
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 767-768 bp 1.0 78.4
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 766-767 bp 1.0 78.4
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 58.1 58.2 787-788 bp 1.0 78.4