Hotspot

rps16-trnQ-UUG

LSC · 5608-7140 · 1533 bp

Back to Chimonobambusa

Pi 0.000356
MarkerSeek score 49.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1533
variable_sites
3
pi
0.000356
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1603-1609 bp 1.0 78.9
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1614-1620 bp 1.0 78.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1607-1613 bp 1.0 77.8
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1618-1624 bp 1.0 77.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAAACCAGAAATGGGGCGT 45.0 45.0 58.1 57.7 1663-1669 bp 1.0 77.1