Hotspot

ndhF

SSC · 109966-112209 · 2244 bp

Back to Chimonanthus

Pi 0.00404

MarkerSeek score 43.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 24
pi: 0.00404
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CATCTGGCAATGGGTAGACC	TCGGAAACAATTACTAAGAATAAGA	55.0	28.0	58.0	54.3	2317-2330 bp	1.0	41.6
ndhF_p2	ATCTGGCAATGGGTAGACCT	TCGGAAACAATTACTAAGAATAAGA	50.0	28.0	58.1	54.3	2316-2329 bp	1.0	41.6
ndhF_p3	CATCTGGCAATGGGTAGACC	AATCGGAAACAATTACTAAGAAT	55.0	26.1	58.0	52.2	2319-2332 bp	1.0	41.6
ndhF_p4	ATCTGGCAATGGGTAGACCT	AATCGGAAACAATTACTAAGAAT	50.0	26.1	58.1	52.2	2318-2331 bp	1.0	41.6
ndhF_p5	TCTGGCAATGGGTAGACCTT	TCGGAAACAATTACTAAGAATAAGA	50.0	28.0	58.3	54.3	2315-2328 bp	1.0	41.6