Hotspot

rps15-ycf1

SSC · 124194-124560 · 367 bp

Back to Chimonanthus

Pi 0.02

MarkerSeek score 46.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 367
variable_sites: 13
pi: 0.02
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TATACGACTCAGCTCCAGCA	ACCACAGGCATTTCATTGGT	50.0	45.0	57.9	58.0	1545-1547 bp	1.0	80.7
rps15-ycf1_p2	CCAATACAGGTTCGCAGTCA	ACCACAGGCATTTCATTGGT	50.0	45.0	57.9	58.0	1345-1347 bp	1.0	80.5
rps15-ycf1_p3	TATACGACTCAGCTCCAGCA	TCCTAGACGTCGCAGAGAAT	50.0	50.0	57.9	58.0	1372-1374 bp	1.0	80.4
rps15-ycf1_p4	TATACGACTCAGCTCCAGCA	TTTTTGGACGAGGACAAGCA	50.0	45.0	57.9	58.0	1257-1259 bp	1.0	80.4
rps15-ycf1_p5	TATACGACTCAGCTCCAGCA	GAGAAATGCGCCTGGATGTA	50.0	50.0	57.9	58.0	1676-1678 bp	1.0	80.3