Hotspot

ccsA-ndhD

SSC · 115963-116196 · 234 bp

Back to Chimonanthus

Pi 0.00855

MarkerSeek score 55.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 234
variable_sites: 6
pi: 0.00855
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTGGCTTCTATGGG	ATCCCGATTTCGTTCTCTCC	50.0	50.0	57.1	57.1	391-416 bp	1.0	71.0
ccsA-ndhD_p2	GGCAATTGTGGCTTCTATGG	ATCCCGATTTCGTTCTCTCC	50.0	50.0	57.1	57.1	392-417 bp	1.0	71.0
ccsA-ndhD_p3	GCAATTGTGGCTTCTATGGG	CCCGATTTCGTTCTCTCCCT	50.0	55.0	57.1	59.2	389-414 bp	1.0	68.8
ccsA-ndhD_p4	GGCAATTGTGGCTTCTATGG	CCCGATTTCGTTCTCTCCCT	50.0	55.0	57.1	59.2	390-415 bp	1.0	68.8
ccsA-ndhD_p5	GCAATTGTGGCTTCTATGGG	TCCCGATTTCGTTCTCTCCC	50.0	55.0	57.1	59.2	390-415 bp	1.0	68.7