Hotspot

rpl32-trnL-UAG

SSC · 113433-114807 · 1375 bp

Back to Chimonanthus

Pi 0.00588

MarkerSeek score 49.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1375
variable_sites: 20
pi: 0.00588
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGCGTCAAAAGCTTTGTCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1493-1498 bp	1.0	80.5
rpl32-trnL-UAG_p2	CGCGTCAAAAGCTTTGTCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1494-1499 bp	1.0	80.5
rpl32-trnL-UAG_p3	CGCGTCAAAAGCTTTGTCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	1503-1508 bp	1.0	79.6
rpl32-trnL-UAG_p4	CGCGTCAAAAGCTTTGTCTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.9	57.8	1502-1507 bp	1.0	79.0
rpl32-trnL-UAG_p5	CGCGTCAAAAGCTTTGTCTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.9	57.8	1501-1506 bp	1.0	79.0