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psbE-petL

LSC · 65743-67024 · 1282 bp

Back to Chimonanthus

Pi 0.00156

MarkerSeek score 54.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 6
pi: 0.00156
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1430-1438 bp	1.0	75.9
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.5	1428-1436 bp	1.0	75.9
psbE-petL_p3	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1435-1443 bp	1.0	75.8
psbE-petL_p4	TGCTGACGAATAACCAACCC	GCAGCCAGTAGAAAACCGAA	50.0	50.0	58.2	58.5	1433-1441 bp	1.0	75.7
psbE-petL_p5	CAAAAGAACGTTCTCCCGTG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1348-1356 bp	1.0	72.0