Hotspot

petA-psbJ

LSC · 63824-64980 · 1157 bp

Back to Chimonanthus

Pi 0.00864

MarkerSeek score 65.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 21
pi: 0.00864
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1295-1334 bp	1.0	73.3
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	45.0	50.0	58.2	56.7	1344-1383 bp	1.0	70.1
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	58.2	56.7	1343-1382 bp	1.0	70.1
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	1302-1341 bp	1.0	68.4
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	45.0	47.6	58.2	58.5	1344-1383 bp	1.0	68.1