Hotspot

ndhC-trnV-UAC

LSC · 51152-52326 · 1175 bp

Back to Chimonanthus

Pi 0.00244

MarkerSeek score 54.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1175
variable_sites: 8
pi: 0.00244
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TTCTCTGGTCCTTCCCGAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	58.0	58.2	1391 bp	1.0	78.8
ndhC-trnV-UAC_p2	TTCTCTGGTCCTTCCCGAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1327 bp	1.0	78.5
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	1370 bp	1.0	78.0
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1306 bp	1.0	77.8
ndhC-trnV-UAC_p5	TTCTCTGGTCCTTCCCGAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.0	57.5	1326 bp	1.0	75.8