Hotspot

psbC-trnS-UGA

LSC · 36203-36422 · 220 bp

Back to Chimonanthus

Pi 0.0176

MarkerSeek score 43.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 11
pi: 0.0176
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCCTATTCGTGGGCCATTTG	TATGGCCATGTTCCATTCGG	50.0	50.0	57.9	58.0	474 bp	1.0	81.6
psbC-trnS-UGA_p2	TCCTATTCGTGGGCCATTTG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	413 bp	1.0	81.6
psbC-trnS-UGA_p3	CGACGTTCCGCGGAATATAT	TATGGCCATGTTCCATTCGG	50.0	50.0	58.0	58.0	613 bp	1.0	81.3
psbC-trnS-UGA_p4	CGACGTTCCGCGGAATATAT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	552 bp	1.0	81.2
psbC-trnS-UGA_p5	TCTAAGGGGTCCCAATGGTT	TATGGCCATGTTCCATTCGG	50.0	50.0	57.9	58.0	677 bp	1.0	80.8