Hotspot

trnT-GGU-psbD

LSC · 32394-33771 · 1378 bp

Back to Chimonanthus

Pi 0.0031

MarkerSeek score 61.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1378
variable_sites: 11
pi: 0.0031
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	55.0	55.0	58.1	57.9	1520-1525 bp	1.0	79.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	50.0	55.0	58.1	57.9	1523-1528 bp	1.0	79.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1528-1533 bp	1.0	78.1
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1531-1536 bp	1.0	77.9
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	55.0	55.0	58.1	58.3	1521-1526 bp	1.0	77.8