Hotspot

atpH-atpI

LSC · 14141-15219 · 1079 bp

Back to Chimonanthus

Pi 0.00173

MarkerSeek score 54.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1079
variable_sites: 5
pi: 0.00173
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1166-1167 bp	1.0	76.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CAAGCGGGATTCAAGCTCTT	45.0	50.0	58.0	58.5	1187-1188 bp	1.0	75.8
atpH-atpI_p3	GATACCTTCTACGGCTTGGC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1251-1252 bp	1.0	75.2
atpH-atpI_p4	CGATACCTTCTACGGCTTGG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	1252-1253 bp	1.0	75.2
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1182-1183 bp	1.0	75.1